A Novel Ratiometric Fluorescent Approach for the Modulation of the Dynamic Range of Lateral Flow Immunoassays

The majority of lateral flow assays (LFAs) use single-color optical labels to provide a qualitative naked-eye detection, however this detection method displays two important limitations. First, the label makes LFA prone results misinterpretation. Second, it does not allow precise modulation sensitivity and dynamic range test. To overcome these limitations, ratiometric approach is developed. In particular, using anti-HIgG functionalized red-fluorescent quantum dots on conjugate pad (as target dependent labels) blue-fluorescent nanoparticles fixed test line independent reporters), possible generate wide color palette (blue, purple, pink, red) line. It believed that strategy will facilitate development LFAs by easily adjusting their analytical properties needs required specific application.